Foodstuffs. Detection of food allergens by molecular biological methods - Celery (Apium graveolens). Detection of a specific DNA sequence in cooked sausages by real-time PCR Ingestion-build-264231 drawings for fire service use

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Foodstuffs. Detection of food allergens by molecular biological methods - Celery (Apium graveolens). Detection of a specific DNA sequence in cooked sausages by real-time PCR Ingestion-build-264231 drawings for fire service use1 Scope This document specifies a method for the detection of celery (Apium graveolens) in emulsion type sausages (e. g. Frankfurter, Wiener). Real time PCR (polymerase chain reaction) detection of celery is based on an 101 bp (base pair) sequence from the gene of the mannitol dehydrogenase (GenBank Acc. No. AF0670821) of celery (Apium graveolens). The method has been validated on emulsion type sausages (Bavarian Leberkse) spiked with celery. For this